Figure 1: Key Predictors of Post-Thaw Viability

<p dir="ltr">For post-thaw viability, each additional day of blood storage prior to cell isolation was associated with a 5.31% decrease in viability (95% CI: –7.24 to –3.37; p = 0.001). Blood stored at room temperature, compared with 4 °C, showed a 5.58% reduction in viability (95% CI: –9.99 to –1.18; p = 0.013). A 1% increase in storage viability corresponded to a 0.26% increase in post-thaw viability (95% CI: 0.10 to 0.42; p = 0.001). Thawing at an external laboratory (non-ENDIA SOP), compared with the ENDIA SOP, reduced viability by 11.50% (95% CI: –21.50 to –1.51; p = 0.024). Shipment in LN2, compared with dry ice, increased viability by 21.10% (95% CI: 13.81 to 28.38; p < 0.001).</p>